Part:BBa_K4343112

TsElo5

TsElo5 encodes ∆-5 elongase from Thraustochytrium sp. which functions in the Δ-9 elongase/desaturase pathway and could convert C20:5 to C22:5.

Description

The TsELO5 gene e ncodes a ∆-5 elongase of 449 amino acids from Thraustochytrium sp. that catalyzes the conversion of Eicosapentaenoic acid (EPA; C20:5n-3) to Docosapentaenoic acid (DPA; C22:5 n- 3).

Literature

Hashimoto et al. employed this enzyme in A. limacinum mh0186 to obtain target product yield of 63%, demonstrating the high availability of the enzyme activity.

Reference

Ohara, J., Sakaguchi, K., Okita, Y., Okino, N., & Ito, M. (2013). Two fatty acid elongases possessing C18-Δ6/C18-Δ9/C20-Δ5 or C16-Δ9 elongase activity in Thraustochytrium sp. ATCC 26185. Marine biotechnology, 15(4), 476-486.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 826
  Illegal XhoI site found at 19
  Illegal XhoI site found at 807
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1
  Illegal BsaI.rc site found at 956